During nucleotide addition, the incoming nucleotide is attached to which part of the growing strand?

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Multiple Choice

During nucleotide addition, the incoming nucleotide is attached to which part of the growing strand?

Explanation:
Nucleotide addition occurs at the free 3' hydroxyl group of the growing strand. DNA polymerase forms a phosphodiester bond between that 3' OH and the incoming dNTP’s phosphate, extending the strand in the 5'→3' direction. The incoming nucleotide donates its phosphate to become linked in the backbone, while the other phosphates are released as pyrophosphate. The sugar, 5' phosphate, or base of the previous nucleotide do not serve as the attachment point for the new nucleotide.

Nucleotide addition occurs at the free 3' hydroxyl group of the growing strand. DNA polymerase forms a phosphodiester bond between that 3' OH and the incoming dNTP’s phosphate, extending the strand in the 5'→3' direction. The incoming nucleotide donates its phosphate to become linked in the backbone, while the other phosphates are released as pyrophosphate. The sugar, 5' phosphate, or base of the previous nucleotide do not serve as the attachment point for the new nucleotide.

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